Phis2图谱

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August undefined, 2024

Webb20 juni 2024 · pHis2 酵母单杂交载体 -- -- 多克隆位点，限制性内切酶 7207bp -- -- -- 卡纳霉素 -- 含HIS3报告基因 -- -- -- -- 载体质粒图谱和多克隆位点信息载体简介载体序列 LOCUS … Webb10 nov. 2015 · 植物表达载体 pcambia1301（EHA105）. 可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯，pQE 载体，原核表达载体，克隆载体，毕赤酵母表达载体，毕赤酵母宿主菌，酿酒酵母表达载体，酿酒 ...

Disruption of the bHLH transcription factor Abnormal Tapetum 1 …

WebbDescription pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ OneHybrid Library Construction & Screening Kit (#K1617-1). Webb多克隆位点的存在可以确保载体合适大部分的dna片段，可以针对插入片段提供特定的酶切位点图谱，在质粒重组操作方面具有更大的灵活性。在选择质粒载体时既要考虑在多接头处出现的是何位点，又要考虑到这个位点的顺序，因为必须确保特定的位点存在于多接头里。 small meeting hotels in southern california

新-酵母单杂交筛选与启动子pHIS2-A互作蛋白结题报告 - 综合文库网

Webb19 nov. 2012 · 是选取atg上游2000的片段，然后整个连到phis2载体上吗？我之前做了直接取ATG上游1.5k构建到phis2上，感觉不合适。最好还是找到自己转录因子的结合motif然后取motif和其上下游几十bp的片段，然后串联3个片段构建载体比较合适。 WebbSnapGene Viewer. SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of … Webb1 dec. 2024 · AD/pATM1-pHIS2 and AD-ATM1/pATM1-pHIS2 were cotransformed into the Y187 strain and cultured on SD/-Trp/-Leu for 2–3 days at 30 °C. Then, healthy and consistent clones were diluted, ... small meeting rooms near me

Phis2图谱

Webb更多详情咨询 www.bnbiotech.com, 021-61496710,15800960770 下面所有质粒和菌株，均为本公司实验室亲自验证过，可以提供技术支持，载体来源于promega,addgene,clontech,novagen,invitrogen可提供质粒图谱，可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真 … http://www.bioon.com.cn/doc/showarticle.asp?newsid=96627

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WebbpHis2载体价格1000元，pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文，载体质粒抗性为Kanamycin，质粒大小为7207bp。pHis2是酵母单杂交载体的pHis2。 Webb1 mars 2024 · C. Yeast one-hybrid assay detect the specific binding ability of VvWRKY30 to W-BOX. pGADT7-empty and pHIS2.1-W-BOX, AD- VvWRKY30 and pHIS2.1-empty were used as negative controls. W boxes have been described as positive cis-acting elements upregulating transcription after binding with WRKY transcription factor.

Webbpwk.baidu.comIS2 质粒图谱载体描述 pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One- Hybrid Library Construction & Screening Kit (#K1617-1). http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html

WebbWith the development of pGADT7-Rec2 (and pHIS2, a low-copy reporter vector), integration is no longer necessary because each plasmid, with its CEN and ARS elements, now behaves like a minichromosome, both mitotically and meiotically. Use. pGADT7-Rec2 is engineered for constructing GAL4 AD/cDNA libraries by homologous recombination in … Webb优宝生物提供pcambia1300载体,产品优质,服务快速,同时提供pcambia1300质粒图谱,载体序列,载体抗性及操作手册等信息 pCAMBIA1300载体_质粒图谱 - 优宝生物如何订购联系我们

Webb12 juli 2024 · 质粒图谱 . 描述： pGBKT7-53 是阳性对照质粒，能编码小鼠p53蛋白（a.a. 72-390 ）和GAL4 DNA-BD（a.a. 1-147 ）的融合蛋白。小鼠p53 c DNA 克隆进入pGBKT7的Eco RI 和Bam H1 位点之间。 p53 c DNA 来源于L wabuchi et al.（1993）年文献提到的质粒（由 C lontech改造所得）。. 保存与运输方法. 保存：-20℃保存，至少1年有效。

http://muchong.com/t-9775583-1 sonnie\u0027s edge short storyWebb1 apr. 2024 · The Y187 strain was prepared for the Y1H assay, we mixed pAD-CmBBX7 and pAD-GUS with pHIS2-CmFTL1pro respectively, and transformed them into Y187 strain using the lithium acetate method.Then inoculated on SD/His-Trp-Leu-plates, and screened for the lowest concentration range of 3-AT that was sufficient to inhibit the growth of … sonni choi williamshttp://www.youbio.cn/product/vt1375 sonnich frylandWebb8 juli 2024 · pHis2 编号载体名称北京华越洋生物VECT2110 pHis2 pHis2 载体基本信息出品公司: Clontech 载体名称: pHis2 质粒类型: 酵母单杂交载体高拷贝/低拷贝: -- 启动子: -- 克隆方法: 多克隆位点，限制性内切酶载体大小: 7207bp 5' 测序引物及序列: -- 3' 测序引物及序列: -- 载体标签 ... small meeting rooms nottinghamWebbpGADT7-Rec2载体图谱： pGADT7-Rec2载体简介： pGADT7-Rec2 is a cloning vector that can be used in yeast to express a protein of interest as a GAL4 activation domain (GAL4 AD) fusion. Transcription starts with the constitutive ADH1 promoter (PADH1) and ends with the ADH1 termination signal (TADH1). sonnicsWebbBiovector,Inc质粒载体菌株细胞株基因保藏中心列表网址h ttp://biovector.blog.163.com 质粒载体索取报价价格优惠售价出售图谱序列酶切位点转化构建求购购买订购 sequence map plasmid vector gene strain order ship 抗性 resistance restriction site BJ5183 small meeting rooms to hire londonWebb载体质粒图谱和多克隆位点信息载体简介 pYES2的是一个5.9 kb的载体，设计用来在酿酒酵母（Saccharomyces cerevisiae）中诱导表达重组蛋白。载体的特点在于基因插入载体 … small meeting room microphone