D10.g4.1 mouse helper t cells

Author: qwdj

August undefined, 2024

WebMeasured by its ability to neutralize IL-1 alpha /IL-1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. ... is typically 0.05-0.15 ug/mL in the presence of 50 pg/mL Recombinant Mouse IL-1 alpha /IL-1F1 and 1.25 ug/mL concanavalin A. Reviewed ... WebED 50 10.0 pg/ml, measured by the dose-dependent stimulation of mouse D10.G4.1 helper T cells, ... keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte ...

重组小鼠 IL-1 alpha / IL1A / IL1F1 蛋白-抗体试剂—环保设备商城

WebD10 G4 1 T Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ... Effects of epalrestat on the cellular AR activity when D10.G4.1 mouse Th2 cells were treated with 10 μM epalrestat. ... Serendipitous Discovery of a Novel Murine Astrovirus Contaminating a Murine Helper T-cell Line and ... WebApr 13, 2024 · Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1 beta /IL … earthgro black mulch home depot

Recombinant Mouse IL-1 alpha/IL-1F1 Protein 400-ML-005: R&D …

WebJun 22, 2024 · Briefly, D10.G4.1 cells (2 x 10 6 cells/well) were dispensed into anti-CD3 mAb coated 6-well plates, with or without varying concentrations of NK-4, and incubated for 6 h at 37°C. Total RNA was extracted from D10.G4.1 cells using an RNeasy Mini kit (QIAGEN, Tokyo, Japan) and DNase (QIAGEN) according to the manufacturer’s … WebWe describe here a stable subclone (D10S) of the murine D10.G4.1 helper T-cell which proliferates to subfemtomolar (attomolar) concentrations of IL-1 beta or alpha in the … WebRat IL-1 alpha/IL-1F1 in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 range is less than or equal to 20 pg/ml. earthgro brown mulch ingredients

IL-1a from rat, Interleukin-1a, recombinant, expressed in E. coli ...

Characterization of a subclone (D10S) of the D10.G4.1 …

WebMouse: Full name is D10G4.1. This is a Th2 helper cell line, derived from AKR/J (H-2K) mice, specific for egg white conalbumin. Tissues: Lymphocyte: Phenotype: Suspension: … WebMeasured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED 50 for this effect is typically 2-10 pg/mL. 2. Measured by its binding ability in a functional ELISA. 3. Immobilized mouse IL1A at 10 μg/mL (100 μl/well) can bind mouse IL1R1, The EC 50 of mouse IL1R1 is 0.13 μg/mL. earthgro by scotts mulchWebIL-1 alpha / IL1A / IL1F1 protein: Bio-activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED 50 for this effect is typically 0.2-1 ng/mL. Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method. Stability: Samples are stable for up to twelve months from date of receipt at -70 ... cth990

"WebGet crucial instructions for accurate ICD-10-CM D47.01 coding with all applicable Excludes 1 and Excludes 2 notes from the section level conveniently shown with each code. This … " - D10.g4.1 mouse helper t cells

D10.g4.1 mouse helper t cells

Recombinant Mouse IL-33 carrier-free - BioLegend

WebMouse IL-33 induces the proliferation of D10.G4.1 mouse T lymphocytes in a dose dependent manner. Description IL-33 belongs to the IL-1 family and is closely related in … WebInterleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory …

Did you know?

WebApr 14, 2024 · the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Human IL-1a/IL-1F1 Membrane Form Monoclonal Antibody (Catalog # MAB2001). The ND50is typically 5-20 µg/mL. … WebRecombinant human IL-1α induces the proliferation of D10.G4.1 mouse helper T lymphocytes in a dose dependent manner. BioLegend’s protein was compared side-by-side to the leading competitor’s equivalent product. ... 86/678) as determined by the dose dependent stimulation of D10.G4.1 cell proliferation. For more information on specific ...

WebRecombinant Human IL-1 alpha/IL-1F1 Protein Bioactivity Recombinant Human IL-1 alpha /IL-1F1 (Catalog # 200-LA) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED 50 for this effect is 1-6 pg/mL. Recombinant Human IL-1 alpha/IL-1F1 Protein SDS-PAGE 1 μg/lane of Recombinant Human IL-1 alpha /IL-1F1 …

WebGeneral. Specific applications. D10.G4.1 has been used extensively to study activation of T cells. These cells can also be used as a reliable and sensitive assay for IL-1 (interleukin-1) response, since D10 cells will … WebEach lot of this antibody is quality control tested by neutralization of proliferation induced by recombinant mouse IL-1β (Cat. No. 575102) at 0.05 ng/mL on D10.G4.1 mouse helper …

WebRight panel: D10.G4.1 (mouse Th2, ATCC TIB -224) cell lysate was probed with Mouse anti-GATA3 monoclonal antibody at concentrations of 0.0625 (lane 1), 0.0156 (lane 2), and 0.0039 µg/ml (lane 3). GATA3 is identified as a band of 50 kDa. MIDDLE LEFT, Immunofluorescent staining of human breast adenocarcinoma.

WebThe biological activity of recombinant rat IL-1α was measured by its ability to stimulate cell proliferation using a mouse helper T cell line, D10.G4.1. Safety Information. Storage Class Code. 13 - Non Combustible Solids. WGK. WGK 3. Flash Point(F) Not applicable. Flash Point(C) Not applicable. cth 95WebAt present, few available drugs can be used to either improve pathological features or prevent the progression of Alzheimer's disease (AD). DL0410 ((1,1'-([1,1'-biphenyl]-4,4' … cth 9983WebNov 4, 2024 · DL0410 has been shown to reverse cognitive impairments in AD animal models, including an Aβ 1–42-induced amnesia mouse model, an APP/PS1 transgenic … cthackframeworkWebD10.G4.1 (mouse, AKR/J(H-2k), lymphocyte, helper T)SPECIES: mouse, AKR/J(H-2k) TISSUE/ORGAN: lymphocyte, helper T DSMZ catalog code: ACC 45 Morphology: single, round cells in suspension Description: mouse T-helper cells clone established from an AKR/J(H-2 k ) mouse; indicator cell line for IL-1 confirmed as mouse with IEF of AST, … cth 9801WebJun 22, 2024 · In our study, however, T-bet mRNA expression was not examined in Fig 5, since T-bet is not expressed in D10.G4.1 cells . NK-4 had little effect on IFN-γ … cth 96072000Webecombinant human IL-1 beta (HZ-1164) stimulates does-dependent proliferation of the D10.G4.1 mouse helper t lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability … earthgro brown mulch scottsWebATCC mouse d10 g4 1 t helper 2 cell Mouse D10 G4 1 T Helper 2 Cell, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed … cth abbreviation in law