WebTemplate concentration was determined with Hoechst dye and a Millipore Cytofluor 2350. DNA concentrations were not adjust- ed, but low-yielding templates (<30 ng/Il) were identified where possible and not se- quenced. Templates were also prepared from two H. influenzae h genomic libraries (20). An amplified library was constructed in vector X ... WebOct 15, 1999 · Fluorescence plate assays were performed in 96-well microtiter plates using a Cytofluor 2350 fluorometer (Millipore/Perseptive Biosystems). Both fluorescein and GFP were measured with a fluorescein filter set (Ex 485±20/Em 530±25) in the plate reader with a sensitivity of 2 on a scale of 10. Flow cytometric analysis of cells was done using ...

Feeding acetyl- l -carnitine and lipoic acid to old rats ...

WebDec 19, 1997 · Fluorescence activity was determined at 485±20-nm excitation and 530±25-nm emission wavelengths using the CytoFluor 2350 fluorescence measurement system (Millipore Corp). Since the cells in the plates were not disturbed by the measuring procedure, the plates could be incubated for various time periods and reread as desired. WebOct 1, 2009 · After further incubation at room temperature for over 2 h under protection from light, culture plates were scanned on a CytoFluor 2350 scanner using the 360/460 nm filter excitation and emission set. The DCF fluorescence units were normalized to the Hoechst-DNA fluorescence units for each well and used as a measure of the level of ROS being ... florist in gulf shores alabama

Molecular Devices Skantron SkanWasher 400

WebMillipore Cytofluor 2350 fluorescent plate reader. The data present-Figure 3. The mitochondria are sources for the detachment-induced rise in ROS. A, Levels of DHR123 fluorescence in plated cells and 30 seconds after cell detachment. B, Levels of DCF fluorescence in suspended cells with or without (–) pretreatment using the mito- Web-300 3150 3100 3050 -100 -200 -300 ioo 50 3000 kilometers 100 200 2950 south scale 000 (l mm = 5 km) at mercator projection 700 2850 800 900 1 WebJan 10, 2000 · Finally, cells were stained with (2′,7′-bis-(2-carboxyethyl)-5-(and-6-)-carboxyfluorescein (BCECF-AM, Molecular Probes, Leiden, The Netherlands) (fc 10 μM) for 30 min and after washing fluorescence activity was detected using the CytoFluor 2350 (Millipore, Bedford, MA) fluorescence measurement system . 2.5. Statistical analyses great workout shoes for women